superscript tm iv reverse transcriptase 18090200  (Thermo Fisher)

Article Title: Uncovering the whole genome silencers of human cells via Ss-STARR-seq
Article Snippet: Subsequently, enrich mRNA using Dynabeads Oligo (dT)25 (Thermo Fisher Scientific, 61002). .. SuperScript TM IV Reverse Transcriptase (Thermo Fisher Scientific; 18090200) and 2 M specific primers (Supplementary Data ) were used for reverse transcription of the enriched mRNA to generate a cDNA product, and then AmbionTM RNase H was used to remove the residual RNA in the cDNA product. .. After purification of the cDNA product, the HiFi high fidelity enzyme Ready Mix (Roche, KK2601) for PCR amplification (Supplementary Data , 98 °C 45 s; after 20 cycles of amplification at 98 °C for 15 s, 60 °C for 30 s, and 72 °C for 30 sec, the products were recovered by 2% agarose gel electrophoresis for 40 min (120 V, 130 mA) to construct the output library for high-throughput sequencing.

Article Title: A novel isoform of Tensin1 promotes actin filament assembly for efficient erythroblast enucleation
Article Snippet: RNA extraction was performed using GeneJET RNA Purification Kit (Thermo Fisher Scientific, K0731) according to manufacturer’s instructions. .. Reverse transcription was performed on equal amounts of total RNA for each sample using SuperScript TM IV Reverse Transcriptase (Thermo Fisher Scientific, 18090050) to synthesize cDNA. .. RT-qPCR was performed using TaqMan TM Fast Advanced Master Mix (Thermo Fisher Scientific, 4444556) in 20 μL reactions using equal amounts of cDNA per sample mixed with pre-designed TaqMan probes (Thermo Fisher Scientific; see Reagents and Tools Table for probe information).

Article Title: Uncovering the whole genome silencers of human cells via Ss-STARR-seq.
Article Snippet: Subsequently, enrichmRNA using Dynabeads Oligo (dT)25 (Thermo Fisher Scientific, 61002). .. SuperScript TM IV Reverse Transcriptase (Thermo Fisher Scientific; 18090200) and 2M specific primers (Supplementary Data 1) were used for reverse transcription of the enriched mRNA to generate a cDNAproduct, and thenAmbionTMRNaseHwasused to remove the residual RNA in the cDNA product. .. After purification of the cDNA product, the HiFi high fidelity enzyme Ready Mix (Roche, KK2601) for PCR amplification (Supplementary Data 1, 98 °C 45 s; after 20 cycles of amplification at 98 °C for 15 s, 60 °C for 30 s, and 72 °C for 30 sec, the products were recovered by 2% agarose gel electrophoresis for 40min (120 V, 130mA) to construct the output library for high-throughput sequencing.

Article Title: Development of isothermal nucleic acid amplification technologies for rapid detection of Porcine Enterovirus-G
Article Snippet: .. PEV-G RNA was subjected to cDNA synthesis using SuperScript TM IV Reverse Transcriptase (Invitrogen by Thermo Fischer Scientific, cat no. 01288215) according to manufacturer’s instructions. ..

Article Title: TDP1 splice-site mutation causes HAP1 cell hypersensitivity to topoisomerase I inhibition.
Article Snippet: The qPCR primers are as follow: PUM1 (F: 5 ′ -CGGGA GATTGCTGGA CATATAA; R: 5 ′ -C AC ATCC ACC A TGAGTTGGT A) and TDP1 (F: 5 ′ -AATGTGCGGA CCA GTTTA G; R: 5 ′ - AA GTCTCA GCTGA CCATTTG). qPCR reactions were prepared in technical triplicates. .. To test the exon skipping, TDP1 mRNA was reverse transcribed with a TDP1 gene-specific primer (5 ′ - AC ATGTTCCC ATGCGTATCC) and the SuperScript TM IV Reverse Transcriptase (Thermo Fisher Scientific). ..

Article Title: Symbiont-specific uptake is mediated by integrins in cnidarian larvae
Article Snippet: RNA was applied to RNeasy Mini Kit columns (Qiagen, 74104) and purified according to manufacturer’s instructions. cDNA was transcribed using the SuperScript ® IV Reverse Transcriptase (InvitrogenTM, 18090010; ThermoFisher Scientific TM ) following manufacturer’s instructions.

Article Title: TDP1 splice-site mutation causes HAP1 cell hypersensitivity to topoisomerase I inhibition
Article Snippet: To test the exon skipping, TDP1 mRNA was reverse transcribed with a TDP1 gene-specific primer (5′-ACATGTTCCCATGCGTATCC) and the SuperScript TM IV Reverse Transcriptase (Thermo Fisher Scientific).

Article Title: Development of isothermal nucleic acid amplification technologies for rapid detection of Porcine Enterovirus-G
Article Snippet: .. PEV-G RNA was subjected to cDNA synthesis using SuperScript TM IV Reverse Transcriptase (Invitrogen by Thermo Fischer Scientific, cat no. 01288215) according to manufacturer’s instructions. ..